E3763

Resorufin ethyl ether

≥98% (TLC), powder

• Synonym(s): 7-Ethoxy-3H-phenoxazin-3-one, Ethoxyresorufin, O⁷-Ethylresorufin
• Empirical Formula (Hill Notation): C₁₄H₁₁NO₃
• CAS Number: 5725-91-7
• Molecular Weight: 241.24
• Beilstein/REAXYS Number: 225973
• MDL number: MFCD00037661
• UNSPSC Code: 12161501
• PubChem Substance ID: 24894527
• NACRES: NA.47

Properties

• Quality Level: 200
• assay: ≥98% (TLC)
• form: powder
• storage condition: (Tightly closed. Dry)
• technique(s): activity assay: suitable
• color: orange to red
• mp: 223-225 °C (lit.)
• solubility: chloroform: 9.80-10.20 mg/mL, clear, orange
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Resorufin ethers are used as markers or substrates for different cytochrome P (CYP) isoforms. Resorufin ethyl ether is the preferred resorufin ether to be metabolized by microsomes of 3-methylcholanthrene treated animals.[1]

Resorufin is the reduced product of resazurin, also termed as Alamar Blu.[2] It exhibits fluorescence at longer wavelengths and is flexible for modification in its hydroxyl group.[3]

Research area: Cell Signaling[3]

Application

Resorufin ethyl ether is suitable for the study of dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, where arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates are used as inhibitors.[4] It is suitable to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1).[5]

Substrates

Fluorimetric substrate for cytochrome P450 linked enzyme, CXPIA1/2

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)