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E9034

Sigma-Aldrich

Phosphodiesterase 5A1 human

recombinant, expressed in Sf9 cells

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Synonym(s):
3′,5′-cyclic-GMP phosphodiesterase, PDE 5A1
Enzyme Commission number:
NACRES:
NA.54

recombinant

expressed in Sf9 cells

Quality Level

form

solution

specific activity

≥2,000 unit/μg protein

mol wt

126 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDE5A(8654)

Application

Phosphodiesterase (PDE) is an enzyme that is used to breaks phosphodiester bonds. PDE5 is a molecular target for the treatment of erectile dysfunction and pulmonary hypertension. PDE5 is used to find novel PDE5 inhibitors .

Biochem/physiol Actions

PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis .

Physical properties

N-terminal GST-tagged 126 kDa full-length protein

Unit Definition

One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C.

Physical form

Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN® 20 and 3 mM DTT

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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