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P9041

Sigma-Aldrich

Phosphodiesterase II from bovine spleen

lyophilized powder, ≥5.0 units/mg protein

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Synonym(s):
3′-Exonuclease, Spleen exonuclease, Spleen phosphodiesterase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥5.0 units/mg protein

mol wt

65 kDa

UniProt accession no.

foreign activity

5′-Nucleotidase ≤1% of base activity

storage temp.

−20°C

Gene Information

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Sigma-Aldrich

E9034

Phosphodiesterase 5A1 human

Sigma-Aldrich

P5511

Protein Kinase A from bovine heart

specific activity

≥5.0 units/mg protein

specific activity

≥1,000 units/mg protein

specific activity

≥2,000 unit/μg protein

specific activity

≥0.8 units/μg protein

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

solution

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

mol wt

65 kDa

mol wt

38 kDa

mol wt

126 kDa

mol wt

-

UniProt accession no.

P14099

UniProt accession no.

-

UniProt accession no.

O76074

UniProt accession no.

P00517, P05131

General description

Phosphodiesterase (PDE) II belong to the PDE superfamily and includes subtypes from one to 11. They exists as a dimer and comprise a N-terminal regulatory domain, C-terminal prenylated domain and a Zn2+ containing catalytic domain. The bovine spleen PDE2 corresponds to a molecular weight of 65 kDa. It requires divalent cations for its enzymatic activity and has an optimum pH of 7.5.

Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase II has been used in the enzymatic digestions of purified proteins such as the P8-dGMP complex. Bovine spleen phosphodiesterase has been used to digest N-cadherin.
Phosphodiesterase II from bovine spleen has been used in the:
  • excision of pyridyloxobutyl (POB) base adducts from DNA
  • digestion of DNA prior to cycloadenosine enrichment
  • hydrolysis of DNA from blue mussels gill tissue into deoxyribonucleoside 3′-monophosphates

The product has been used in the characterization of polynucleotide chain length, base composition, and identity of terminal nucleotide. The enzyme has also been used in excision of pyridyloxobutyl (POB) base adducts from DNA. Furthermore, it has been used along with micrococcal endonuclease to hydrolyze purified DNA to 3 -nucleoside monophosphates.

Biochem/physiol Actions

Phosphodiesterase (PDE) breaks phosphodiester bonds. 3-Isobutyl-methylxanthine (IBMX) is a potential PDE2 inhibitor.
The enzyme acts on poly(A), poly(U), and poly(I). Native DNA and poly(C) are quite resistant to the action of this enzyme.
Hydrolyzes RNA, RNA-Core, 3′-alkyl- and 3′-aryl-nucleoside phosphates, and polydeoxyribonucleotides with 3′-phosphate end groups to 3′-mononucleotides.
Polynucleotides having 5′-phosphomonoester end groups are not attacked.

Unit Definition

One unit will produce acid soluble nucleotides equivalent to a ΔA260 of 16 in 30 min at pH 6.5 at 37 °C, in a 2.0 mL reaction mixture. Substrate: RNA-Core. Actual A260 is measured on the supernatant after precipitation of the unhydrolyzed RNA with uranyl acetate-perchloric acid reagent.

Analysis Note

Protein determined by biuret

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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