N8630
Nuclease P1 from Penicillium citrinum
lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
Recommended Products
biological source
Penicillium citrinum
Quality Level
form
lyophilized powder
specific activity
≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
secondary activity
≥1,000 units/mg protein 3′-nucleotidase
mol wt
42-50 kDa
packaging
vial of ≥250 units (using RNA substrate)
technique(s)
DNA extraction: suitable
DNA purification: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
storage temp.
2-8°C
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General description
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure.
Application
- Nuclease P1 cleave of single stranded DNA or RNA to 5′ mononucleotides
- Nuclease P1 supports DNA damage and modification research
- Nucleic acids base composition and structural analysis can be done by Nuclease P1
- Nuclease P1 has historically been used for the industrial production of 5′-mononucleotides from yeast RNA.
- Removal of nucleic acids through protein purification can be done by Nuclease P1
- Nuclease P1 is a key reagent for the development of methods for studies involving t-RNA dependent amino acid biosynthesis and t-RNA dependent trans-amidation
- Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent.
- Nuclease P1 was used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA.
- Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
Biochem/physiol Actions
Features and Benefits
Physical properties
Unit Definition
3′-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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