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F4137

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL number:
MFCD00162224
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
indirect immunofluorescence: 1:200

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .

Specificity

Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.

Immunogen

Chicken IgY (IgG)
Purified chicken IgY (IgG)

Application

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used:
  • in indirect immunofluorescence
  • in western blotting
  • in immunocytochemistry

Biochem/physiol Actions

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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