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F6926

Sigma-Aldrich

Fibroblast Growth Factor-8b from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

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Synonym(s):
FGF-8b
MDL number:
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

15-60 ng/mL

mol wt

calculated mol wt 23.6 kDa
predicted mol wt 22 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Fgf8(14179)

General description

Fibroblast growth factor 8 (FGF8) is a member of the FGF family, and was initially cloned from androgen-dependent mouse mammary carcinoma cell line. Alternative splicing of this gene produces four different isoforms mainly, FGF8a, FGF8b, FGF8e and FGF8f.

Application

Fibroblast Growth Factor-8b from mouse has been used-
  • for subgerminal cavity injection in fertilized quail, chicken, turtle and emu eggs
  • in the induction of neural differentiation in stem cells from Human Exfoliated Deciduous teeth (SHED) and
  • in experiments conducted on fertilized hens′ eggs, where FGF8 induced SOX3 gene.

Biochem/physiol Actions

FGF-8b is involved in ectodermal differentiation in the post-gastrulation embryo, including outgrowth and patterning of the face, limbs, and central nervous system. FGF-8b activates the "c" splice form of FGFR-2, FGFR-3, and FGFR-4.
Fibroblast growth factor 8b (FGF8b) isoform of FGF8 has the most potent angiogenic and tumorigenic properties. In prostate cancer, it is responsible for angiogenesis, tumorigenesis and invasion. Exogenous expression of this gene in prostate epithelium leads to prostatic intraepithelial neoplasia (PIN). It also interacts with pattern recognition receptor long pentraxin-3 (PTX3), which is involved in local inflammation.

Physical form

Lyophilized from a 0.2μm filtered solution in 20mM MOPS, 0.1M sodium sulfate, 0.02% Brij-35, pH 7 containing 50ug of BSA per 1μg as a carrier protein

Analysis Note

The biological activity is measured by its ability to stimulate proliferation of NR6R-3T3 fibroblasts.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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