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F9006

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate
MDL number:
NACRES:
NA.46

biological source

goat

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:64

storage temp.

2-8°C

target post-translational modification

unmodified

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F0257F2012F4018
technique(s)

direct immunofluorescence: 1:64

technique(s)

direct immunofluorescence: 1:32

technique(s)

direct immunofluorescence: 1:64 (minimum)

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

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General description

IgG antibody consists of four subclasses : IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa respectively. The chains are interlinked with a disulfide bond.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for mouse IgG. Goat anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. Following conjugation, unbound FITC is removed by extensive dialysis.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)−FITC antibody produced in goat has been used in flow cytometry.
The antibody may be used for immunofluorescent staining of mouse spleen cells at a working dilution of 1:64. A working antibody dilution of 1:40 was used to stain the conidia of Botrytis cinerea for immunofluorescence. The antibody was also used for immunofluorescence of primary luteal cell cultures incubated with primary antibody against CD45 and rhizoids of Chara globularis incubated with anti-human spectrin antibody.

Biochem/physiol Actions

IgG (immunoglobulin G) antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Specific IgG for cat allergens in patients with allergic conjunctivitis
The Laboratory Rat, 35(4), 575-586 (2005)
Veronica Lanza Cariccio et al.
mAbs, 8(4), 741-750 (2016-03-11)
There is a strong need for rapid and reliable epitope mapping methods that can keep pace with the isolation of increasingly larger numbers of mAbs. We describe here the identification of a conformational epitope using Phage-based Representation OF ImmunoLigand Epitope
Development of a monoclonal antibody-based immunodetection assay for Botrytis cinerea
Bossi R and Dewey FM
Plant Pathology, 41, 472-482 (1992)
Discoidin domain receptors promote alpha1beta1-and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation
Xu H, et al.
PLoS ONE, 7(12), e52209-e52209 (2012)
N C Crespo et al.
Cell death and differentiation, 9(7), 702-709 (2002-06-12)
Recently, we have shown that the farnesyltransferase inhibitor FTI-2153 induces accumulation of two human lung cancer cell lines in mitosis by inhibiting bipolar spindle formation during prometaphase. Here we investigate whether this mitotic arrest depends on transformation, Ras and/or p53

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