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G4416

Sigma-Aldrich

Anti-GABAA Receptor (α1 subunit) antibody produced in rabbit

affinity isolated antibody

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MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

mol wt

antigen ~50 kDa

species reactivity

mouse, rat, human (predicted), bovine (predicted), chicken (predicted)

technique(s)

immunohistochemistry: suitable using rat brain sections
western blot: 1:400-1:1,000 using rat brain membranes

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... GABRA1(2554)
mouse ... Gabra1(14394)
rat ... Gabra1(29705)

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This Item
G0295G0545G5544
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

immunohistochemistry: suitable using rat brain sections, western blot: 1:400-1:1,000 using rat brain membranes

technique(s)

western blot: 1:1000 using rat brain membrane fraction.

technique(s)

western blot: 1:200 using rat brain membrane

technique(s)

western blot: 1:200 using rat brain membranes

mol wt

antigen ~50 kDa

mol wt

-

mol wt

-

mol wt

-

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General description

GABAA and GABAB receptors differ with regard to their ionic characteristics and pharmacological properties. The GABAA receptor is an ionotropic receptor that forms the GABA gated chloride channel and consists of several heterogeneous subunits with membrane recognition sites for benzodiazapenes.

Immunogen

peptide corresponding to amino acids 28-43 of rat GABA(A) receptor α1 subunit.

Application

Anti-GABAA Receptor (α1 subunit) antibody produced in rabbit is suitable for detection of GABA-A α1 subunit by immunohistochemistry using rat brain sections and by immunoblotting at a working dilution of 1:400-1:1000 using rat brain membranes.

Biochem/physiol Actions

The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABAA and GABAB. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. α1 is a subtype of GABAA receptor expressed selectively by serotonergic and GABAergic neurons. Serotonergic neurons express strong α3 immunoreactivity but, do not show any α1 immunoreactivity. On the contrary, GABAergic neurons express both α1 and α3 subunits.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide.

Preparation Note

Reconstitute the 0.05 mL vial with 50 μL deionized water. Reconstitute the 0.2 mL vial with 0.2 mL deionized water. After reconstitution, the antibody concentration is ~0.8 mg/mL.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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