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G6046

Sigma-Aldrich

Anti-G3BP antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-G3BP1, Anti-GAP SH3 domain-binding protein, Anti-HDH-VIII, Anti-Ras-GTPase-activating protein SH3-domain binding protein 1, Anti-human DNA helicase VIII
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~68 kDa

species reactivity

human, mouse (predicted), rat (predicted)

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 0.1-0.2 μg/mL using whole extract of human U-2-OS cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... G3BP1(10146)
mouse ... G3bp1(27041)
rat ... G3bp1(171092)

General description

G3BP is a RNA-binding protein that contains a RNA binding domain, the RRM-type domain, at the carboxyl-terminal. The N-terminal domain is homologous to nuclear transporter factor 2 (NTF2) and the central domain is rich in acidic residues. The RRM domain is involved in the binding of G3BP to specific RNA sequences. This helps G3BP to function as a CA dinucleotide-specific endoribonuclease. G3BP′s subcellular localization and protein-protein interactions are mediated by the phosphorylation of G3BP at Ser149, near NTF2-like domain. G3BP is found in abundance in many kinds of malignant tumors such as lung, colon, gastric, and breast cancer.

Application

Anti-G3BP antibody produced in rabbit has been used in co-immunoprecipitation assay.
Anti-G3BP antibody produced in rabbit has been used in
  • immunofluorescence analysis
  • immunostaining
  • immunoblotting
  • immunoprecipitation

Biochem/physiol Actions

G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) is a endoribonuclease that is phosphorylation-dependent and specific to single strand. It cleaves between cytosine and adenine (CA). It binds to the SH3 domain of RasGAP. It forms a link between RasGAP-mediated signaling pathway and RNA turnover. G3BP is required for the assembly of stress granules (SGs), which are involved in the regulation of mRNA metabolism during stress. It may be used as a cytoplasmic SG marker.
Ras-GAP SH3 domain binding protein (G3BP) is overexpressed in many kinds of malignant tumors such as breast, colon, lung and gastric breast cancer. G3BP expression is correlated with lymph node metastasis in breast cancer.

Target description

G3BP encodes one of the DNA-unwinding enzymes which prefers partially unwound 3′-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

In immunoblotting assays, it is highly recommended to dilute the antibody in 5% NFDM (w/v).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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