G8420
β-Glucuronidase from Escherichia coli
≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
About This Item
Recommended Products
recombinant
expressed in E. coli overproducing strain
Quality Level
form
lyophilized powder
specific activity
≥20,000 units/mg protein
mol wt
290 kDa
composition
, ~25%
storage temp.
−20°C
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Application
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Quality
Unit Definition
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
Protocols
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
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