G9282
Monoclonal Anti-Glutamate antibody produced in mouse
clone GLU-4, ascites fluid
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GLU-4, monoclonal
contains
15 mM sodium azide
species reactivity
wide range
technique(s)
indirect ELISA: 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GRM1(2911)
Specificity
The antibody reacts specifically with L-glutamate (L-glutamic acid), when immobilized with glutaraldehyde. Cross-reaction is observed with Gly-Glu and Asp-Glu. When using the product at the recommended working dilution, in indirect and competitive ELISA, weak cross-reaction may be obtained with D-glutamate, L-glutamine, L-aspartate, D-aspartate, L-asparagine, β-alanine, glycine, 5-aminovaleric acid, γ-aminobutyric acid (GABA) and Gly-Asp. Glutamate is found in cell bodies, axons, and terminals of glutamatergic neurons in the CNS.
Immunogen
L-glutamic acid (Glu).
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)
Immuno-electron microscopy (1 paper)
Monoclonal Anti-Glutamate antibody produced in mouse is suitable for:
- immunolabeling of glutamate in the lamina of the Drosophila visual system
- immunocytochemistry at a working dilution of 1:20,000 to detect glutamate using heart and extrinsic nerves of stomatopod crustacean, Squilla oratoria
- immunofluorescent staining as a primary antibody at a working dilution of 1:1000 using cryosections of brains and embryos from mice
- use as a primary antibody to measure the glutamate release in synaptosome preparations
Biochem/physiol Actions
Glutamate is an amino acid that acts as a excitatory neurotransmitter in the vertebrate brain and functions in all brain processes. It is released from one end of the neuron and it binds to receptors on the surface of adjacent neurons. Glutamate is released in a calcium-dependent manner in response to depolarizing stimuli. It serves as a substrate in certain metabolic pathways, such as, synthesis of glutamine and γ-aminobutyric acid (GABA) Excessive release of glutamate or aspartate have been observed in the pathogenesis of certain neurodegenerative diseases, such as, Huntington′s disease, Alzheimer′s disease. The same is also observed in selective neuronal degenerations involved in epilepsy, ischemia, and hypoglycemia.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A lot of "excitement' about neurodegeneration.
R Taylor
Science (New York, N.Y.), 252(5011), 1380-1381 (1991-06-07)
Ling-Qiang Zhu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(45), 12211-12220 (2007-11-09)
Activation of glycogen synthase kinase-3 (GSK-3) can cause memory deficits as seen in Alzheimer's disease, the most common age-associated dementia, but the mechanism is not understood. Here, we found that activation of GSK-3 by wortmannin or transient overexpression of wild-type
Hiroshi Ando et al.
The Journal of experimental biology, 207(Pt 26), 4663-4677 (2004-12-08)
The heart of Squilla oratoria contains a cardiac ganglion that consists of 15 intrinsic neurons, supplied by a pair of inhibitory nerves and two pairs of excitatory nerves, arising from the central nervous system. These comprise the extrinsic cardiac innervation.
Yun Zhang et al.
The journal of headache and pain, 21(1), 72-72 (2020-06-12)
Vestibular migraine has recently been recognized as a novel subtype of migraine. However, the mechanism that relate vestibular symptoms to migraine had not been well elucidated. Thus, the present study investigated vestibular dysfunction in a rat model of chronic migraine
Agata Kolodziejczyk et al.
PloS one, 3(5), e2110-e2110 (2008-05-10)
Synaptic connections of neurons in the Drosophila lamina, the most peripheral synaptic region of the visual system, have been comprehensively described. Although the lamina has been used extensively as a model for the development and plasticity of synaptic connections, the
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