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GE17-0403-03

HiTrap® Protein A High Performance

Cytiva 17-0403-03, pack of 5 × 5 mL

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Synonym(s):
HiTrap Protein A HP, high performance column, antibody purification
UNSPSC Code:
23151816
NACRES:
NA.56

ligand

native protein A

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-0403-03

storage condition

(20% Ethanol)

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

size

avg. part. size

34 μm

cleaning

2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9

storage temp.

2-8°C

Related Categories

General description

HiTrap® Protein A HP is a prepacked Protein A Sepharose High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient formulation.

Features and Benefits

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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