GE17-3712-20
HIS Mag Sepharose™ Excel
Cytiva 17-3712-20, pack of 2 × 1 mL
About This Item
Recommended Products
ligand
IMAC immobilized with Ni
packaging
pack of 2 × 1 mL
manufacturer/tradename
Cytiva 17-3712-20
parameter
Ambient temp. range
matrix
highly cross-linked spherical agarose including magnetite
General description
Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.
The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.
Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.
Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.
Features and Benefits
- Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
- Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
- Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
- Simplify handling using visible and dense Sepharose-based magnetic beads
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Protocols
This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.
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