GE17-3712-06

HisTrap^™ Excel

Cytiva 17-3712-06, pack of 5 × 5 mL

• Synonym(s): affinity resins, Ni Sepharose excel column, ion affinity chromatography column, IMAC column
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• packaging: pack of 5 × 5 mL
• manufacturer/tradename: Cytiva 17-3712-06
• parameter: 5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.); 5 ml/min - 20 ml/min flow rate (2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.)
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: highly cross-linked 6% agarose
• average diameter: 90 μm
• cleaning: 2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
• working range: 2-13
• capacity: ≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL^® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)

Description

General description

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

Application

Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose^™ excel medium resistant to chelating and reducing agents

Features and Benefits

• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
• Increase target protein yield and decrease degradation through reduced and simplified sample handling
• HisTrap excel columns allow direct purification of cell-free, unclarified samples
• HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
• HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS09
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501