Documents
GE17-3712-06
HisTrap™ Excel
Cytiva 17-3712-06, pack of 5 × 5 mL
Recommended Products
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-3712-06
parameter
5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.)
5 ml/min - 20 ml/min flow rate (2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.)
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
average diameter
90 μm
cleaning
2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
working range
2-13
capacity
≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)
Related Categories
1 of 4
This Item | GE29-0485-86 | GE17-5248-02 | GE17-5248-01 |
|---|---|---|---|
| matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose |
| shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| manufacturer/tradename Cytiva 17-3712-06 | manufacturer/tradename Cytiva 29-0485-86 | manufacturer/tradename Cytiva 17-5248-02 | manufacturer/tradename Cytiva 17-5248-01 |
| parameter 5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.) | parameter 1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.), 5 bar | parameter 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature), 42 psi (H2O at room temperature.) | parameter 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature), 42 psi (H2O at room temperature.) |
| bed size 16 mm × 25 mm | bed size 7 mm × 25 mm | bed size 16 mm × 25 mm | bed size 16 mm × 25 mm |
General description
Application
Features and Benefits
- HiTrap® columns prepacked with Ni Sepharose™ excel medium, resistant to chelating and reducing agents
• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity - Increase target protein yield and decrease degradation through reduced and simplified sample handling
- HisTrap™ excel columns allow direct purification of cell-free, unclarified samples.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.
Difficulty Finding Your Product Or Lot/Batch Number?
How to Find the Product Number
Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).
Example:
Additional examples:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
enter as 1.000309185)
Having trouble? Feel free to contact Technical Service for assistance.
How to Find a Lot/Batch Number for COA
Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.
Not Finding What You Are Looking For?
In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.
Customers Also Viewed
Articles
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Protocols
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.
How to isolate proteins from inclusion bodies using Cytiva products.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service