GE28-9365-51

Hisprep^™ Fast Flow 16/10

Cytiva 28-9365-51

• Synonym(s): 16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9365-51
• parameter: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• bed size: 16 mm × 100 mm
• bed volume: 20 mL
• column I.D.: 16 mm
• matrix: 6% cross-linked agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• capacity: ~40 mg binding capacity(histidine-tagged protein)
• suitability: suitable for bioprocess medium

Description

General description

HisPrep^™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep^™ FF 16/10 columns are prepacked with Ni Sepharose^™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose^™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

Features and Benefits

• Optimized for convenient scale-up purification of histidine-tagged proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible leakage of Ni²⁺.
• High binding capacity, approx. 40 mg/mL medium.
• Convenient and time-saving 20 mL prepacked HiPrep^™ format.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva

HisPrep is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS09
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids