Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
pack of 5 × 1 mL
Cytiva 17-3712-05
1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.)
5 bar
7 mm × 25 mm
1 mL
7 mm
highly cross-linked 6% agarose
90 μm
2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
2-13
≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)
1 of 4
This Item | GE29-0485-86 | GE17-3712-03 | GE29-0510-21 |
---|---|---|---|
matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose |
shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
manufacturer/tradename Cytiva 17-3712-05 | manufacturer/tradename Cytiva 29-0485-86 | manufacturer/tradename Cytiva 17-3712-03 | manufacturer/tradename Cytiva 29-0510-21 |
parameter 1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.) | parameter 1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.), 5 bar | parameter - | parameter flow rate, 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature), 42 psi (H2O at room temperature.) |
bed size 7 mm × 25 mm | bed size 7 mm × 25 mm | bed size - | bed size 7 mm × 25 mm |
Warning
3 - Flammable liquids
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This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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