GE17-3712-05
HisTrap™ Excel
Cytiva 17-3712-05, pack of 5 × 1 mL
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-3712-05
parameter
1 ml/min - 4 ml/min flow rate
5 bar
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
average diameter
90 μm
cleaning
2-14
working range
2-13
capacity
≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)
Related Categories
General description
Features and Benefits
- Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- HisTrap excel columns allow direct purification of cell-free, unclarified samples
- HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
- HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.
Storage and Stability
Analysis Note
Other Notes
Legal Information
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Certificates of Analysis (COA)
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Protocols
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.
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This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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