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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9782-44
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
suitability
suitable for bioprocess medium
Related Categories
1 of 4
This Item | GE28-9782-45 | GE28-9505-13 | GE28-9505-10 |
|---|---|---|---|
| packaging pkg of 1 ea | packaging pkg of 1 ea | packaging pkg of 1 ea | packaging pkg of 1 ea |
| manufacturer/tradename Cytiva 28-9782-44 | manufacturer/tradename Cytiva 28-9782-45 | manufacturer/tradename Cytiva 28-9505-13 | manufacturer/tradename Cytiva 28-9505-10 |
| parameter 1.5 bar (22 psi) (Over the Packed Bed During Operation) | parameter 1.5 bar (22 psi) (Over the Packed Bed During Operation) | parameter 1.5 bar (22 psi) (Over the Packed Bed During Operation) | parameter 1.5 bar (22 psi) (Over the Packed Bed During Operation) |
| bed size 7.7 mm × 100 mm | bed size 7.7 mm × 100 mm | bed size 7.7 mm × 100 mm | bed size 7.7 mm × 100 mm |
| bed volume 4.7 mL | bed volume 4.7 mL | bed volume 4.7 mL | bed volume 4.7 mL |
General description
Application
HiScreen™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose™ 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.
Features and Benefits
- High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
- Prepacked, ready-to-use columns for convenient process development.
- Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
- Easy connection in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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Articles
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Protocols
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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