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HiTrap® Capto S

Cytiva 17-5441-22, pack of 5 × 1 mL

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sulfonate group


Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pack of 5 × 1 mL


Cytiva 17-5441-22


<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm


highly cross-linked agarose with dextran surface extender

average diameter

90 μm (d50v)



working range



suitable for bioprocess medium

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General description

HiTrap® Capto S is a strong cation exchanger prepacked with BioProcess Capto media for screening and small-scale protein purifications using ion exchange chromatography (IEX).


Capto S has a strong sulfoethyl cation exchanger coupled to a chemically modified, high-fLow Agarose matrix. The modified Agarose matrix provides greater particle rigidity without compromising pore size, which allows more flexibility in process-scale applications. High dynamic binding capacity is achieved with a Dextran surface extender that coats the Agarose matrix.

Features and Benefits

  • High-throughput medium for capture and intermediate purification, excellent choice for process-scale purification.
  • The combined properties of high binding capacity, high flow rate, and Low back pressure reduces process cycle times and improves productivity at process scale.
  • Rigid Agarose matrix enables high bed heights and purification of viscous samples at high flow rates.
  • Strong sulfoethyl(S) cation exchanger.

Storage and Stability

Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

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Legal Information

Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva






Storage Class

3 - Flammable liquids

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