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GE29-0513-25

HiTrap® Q HP

Cytiva 29-0513-25, pack of 1 mL

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UNSPSC Code:
23151816
NACRES:
NA.56

ligand

quaternary amine

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0513-25

availability

not available in North America

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

1-14

working range

2-12

suitability

suitable for bioprocess medium

Related Categories

General description

HiTrap Q HP are prepacked, ready-to-use Q Sepharose High Performance strong anion exchange columns for high-resolution, small-scale protein purification.

Application

Separation of protein using a strong anion exchange medium prepacked in the HiTrap column format.

Features and Benefits

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong quaternary ammonium (Q) anion exchanger.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Resource™ Q Cytiva 17-1177-01, pack of 1 mL

GE17-1177-01

Resource Q

Articles

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service