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GE27-0843-01

PreScission Protease

Cytiva 27-0843-01

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Synonym(s):
fusion protein, GST-tag removal
NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 500 units

manufacturer/tradename

Cytiva 27-0843-01

shipped in

dry ice

storage temp.

−20°C

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This Item
SAE0110SAE0045SAE0067
PreScission Protease Cytiva 27-0843-01

GE27-0843-01

PreScission Protease

HRV-3C Protease. N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Sigma-Aldrich

SAE0045

HRV-3C Protease.

SUMO Protease His tagged recombinant protein, lyophilized powder

Sigma-Aldrich

SAE0067

SUMO Protease

manufacturer/tradename

Cytiva 27-0843-01

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

packaging

pkg of 500 units

packaging

-

packaging

-

packaging

-

General description

PreScission Protease is a genetically engineered fusion protein of human rhinovirus 3C protease and glutothione S trasferase (GST). PreScisson protease specifically cleaves between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.

Application

Specific, low temperature cleavage of fusion protein tags; Simple on-column cleavage during affinity purification
PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation. It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

pGEX Vectors
pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Zhen Wang et al.
Nature communications, 10(1), 3162-3162 (2019-07-20)
HECT E3 ligases control the degradation and functioning of numerous oncogenic/tumor-suppressive factors and signaling proteins, and their activities must be tightly regulated to prevent cancers and other diseases. Here we show that the Nedd4 family HECT E3 WWP1 adopts an
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Several bacterial pathogens produce nucleotidyl cyclase toxins to manipulate eukaryotic host cells. Inside host cells they are activated by endogenous cofactors to produce high levels of cyclic nucleotides (cNMPs). The ExoY toxin from Pseudomonas aeruginosa (PaExoY) and the ExoY-like module
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The post-translational modification of proteins with ubiquitin (Ub) and Ub-like modifiers (Ubls) represents one of the most important regulators in eukaryotic biology. Polymeric Ub/Ubl chains of distinct topologies control the activity, stability, interaction and localization of almost all cellular proteins

Articles

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

See All

Protocols

pGEX Vectors

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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