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GE17-5279-02

Glutathione Seph High Performance

Cytiva 17-5279-02, pack of 100 mL

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NACRES:
NA.56

ligand

glutathione

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-5279-02

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

3-12

working range

3-12

capacity

>7 mg/mL binding capacity(medium)

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vibrant-m

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GSTrap High Performance

vibrant-m

GE17-5282-01

GSTrap High Performance

manufacturer/tradename

Cytiva 17-5279-02

manufacturer/tradename

Cytiva 17-5281-01

manufacturer/tradename

Cytiva 17-5282-01

manufacturer/tradename

Cytiva 17-5113-01

capacity

>7 mg/mL binding capacity(medium)

capacity

>7 mg/mL binding capacity(H2O at room temperature.)

capacity

>7 mg/mL binding capacity (medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

capacity

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

packaging

pack of 100 mL

packaging

pack of 5 × 1 mL

packaging

pack of 1 × 5 mL

packaging

pack of 5 mL

ligand

glutathione

ligand

glutathione

ligand

glutathione

ligand

-

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

General description

Glutathione Sepharose High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

Application

Glutathione Sepharose High Performance is used for the purification of GST-tagged Proteins from bacterial lysates and other S-transferases or glutathione-dependent Proteins. GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function.

Features and Benefits

  • 34 μm bead size for improved efficiency and resolution
  • Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
  • Mild conditions during the purification process preserves Protein antigenicity and function

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


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Articles

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

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Protocols

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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