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GE17-0756-05

Glutathione Sepharose 4B

Cytiva 17-0756-05, pack of 100 mL

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Synonym(s):
affinity chromatography resin, GST-tagged proteins purification
NACRES:
NA.56

ligand

glutathione

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-0756-05

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)

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Glutathione Sepharose™ 4B Cytiva 17-0756-05, pack of 100 mL

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Glutathione Sepharose 4B

Glutathione Sepharose™ 4B Cytiva 17-0756-01, pack of 10 mL

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Glutathione Sepharose 4B

Gelatin-Sepharose™ 4B Cytiva 17-0956-01, pack of 25 mL

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manufacturer/tradename

Cytiva 17-0756-05

manufacturer/tradename

Cytiva 17-0756-01

manufacturer/tradename

Cytiva 17-5132-02

manufacturer/tradename

Cytiva 17-0956-01

matrix

4% agarose

matrix

4% agarose

matrix

highly cross-linked 4% agarose

matrix

4% agarose

particle size

45-165 μm

particle size

45-165 μm

particle size

-

particle size

45-165 μm

capacity

>25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)

capacity

>25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)

capacity

>10 mg/mL binding capacity(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)

capacity

-

ligand

glutathione

ligand

glutathione

ligand

glutathione and 10-carbon linker arm

ligand

bovine gelatin

General description

Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Features and Benefits

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

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Sepharose™ 4B 45-165 μm bead diameter

Sigma-Aldrich

4B200

Sepharose 4B

Sajjan Koirala et al.
Cell reports, 32(3), 107922-107922 (2020-07-23)
Spatiotemporal control of Wnt/β-catenin signaling is critical for organism development and homeostasis. The poly-(ADP)-ribose polymerase Tankyrase (TNKS1) promotes Wnt/β-catenin signaling through PARylation-mediated degradation of AXIN1, a component of the β-catenin destruction complex. Although Wnt/β-catenin is a niche-restricted signaling program, tissue-specific

Articles

This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.

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Protocols

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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