GE17-0756-05
Glutathione Sepharose™ 4B
Cytiva 17-0756-05, pack of 100 mL
Synonym(s):
affinity chromatography resin, GST-tagged proteins purification
About This Item
Recommended Products
ligand
glutathione
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0756-05
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)
Related Categories
General description
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
Storage and Stability
Analysis Note
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Warning
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Certificates of Analysis (COA)
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Articles
This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
Protocols
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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