GE17-5132-02

Glutathione Sepharose^™ 4 Fast Flow

Cytiva 17-5132-02, pack of 100 mL

• Synonym(s): Sepharose 4 Fast Flow
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• ligand: glutathione and 10-carbon linker arm
• packaging: pack of 100 mL
• manufacturer/tradename: Cytiva 17-5132-02
• matrix: highly cross-linked 4% agarose
• average diameter: 90 μm
• cleaning: 3-12
• working range: 3-12
• capacity: >10 mg/mL binding capacity

Description

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

Glutathione Sepharose^™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Glutathione Sepharose^™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose^™ 4 Fast Flow provides good flow properties and is excellent for scaling up.

Features and Benefits

• Fast, one-step purification of GST-tagged proteins
• Mild elution conditions preserving protein antigenicity and function
• For scale-up due to high flow properties
• Glutathione coupled to Sepharose^™ 4 Fast Flow via a 10-carbon spacer arm

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids