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G4510

Millipore

Glutathione−Agarose

lyophilized powder

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Synonym(s):
GSH-agarose, S-linked glutathione agarose
MDL number:
NACRES:
NA.56

form

lyophilized powder

Quality Level

analyte chemical class(es)

proteins (GST)

extent of labeling

≥10 μmol per mL gel

technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

matrix

4% cross-linked beaded agarose

matrix activation

epoxy

matrix attachment

sulfur

matrix spacer

12 atoms (10 carbon)

storage temp.

−20°C

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This Item
A8580SAB4200692G3907
Glutathione−Agarose lyophilized powder

Millipore

G4510

Glutathione−Agarose

Millipore

Millipore

G3907

Glutathione−Agarose

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable

technique(s)

ELISA: suitable, dot blot: suitable, immunoblotting: 0.5 μg/mL, immunofluorescence: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

matrix

4% cross-linked beaded agarose

matrix

-

matrix

-

matrix

cross-linked 4% beaded agarose

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

analyte chemical class(es)

proteins (GST)

analyte chemical class(es)

-

analyte chemical class(es)

-

analyte chemical class(es)

proteins (GST)

extent of labeling

≥10 μmol per mL gel

extent of labeling

-

extent of labeling

-

extent of labeling

-

General description

Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.

Application

Glutathione-Agarose has been used:
  • to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
  • to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
  • in affinity purification of the fusion protein

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Biochem/physiol Actions

Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.

Features and Benefits

  • Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
  • Has a shelf life of two years if stored properly desiccated at -20 °C.
  • Binding capacity: 5-10 mg glutathione S-transferase per mL resin

Quantity

One g powder swells to ≥10 ml of gel

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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