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G3907

Millipore

Glutathione−Agarose

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

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Synonym(s):
GSH-agarose, S-linked glutathione agarose
MDL number:
NACRES:
NA.56

Quality Level

form

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

analyte chemical class(es)

proteins (GST)

packaging

set of 3 pre-packed columns (2.5 ml each)

technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

matrix

cross-linked 4% beaded agarose

storage temp.

2-8°C

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This Item
G4510AGRLE-RO69670-M
vibrant-m

G3907

Glutathione−Agarose

vibrant-m

G4510

Glutathione−Agarose

vibrant-m

AGRLE-RO

Agarose LE

vibrant-m

69670-M

His•Bind® Resin

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

-

technique(s)

-

packaging

set of 3 pre-packed columns (2.5 ml each)

packaging

-

packaging

pkg of 100 g (11685660001), pkg of 500 g (11685678001)

packaging

-

form

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

form

lyophilized powder

form

powder

form

liquid

analyte chemical class(es)

proteins (GST)

analyte chemical class(es)

proteins (GST)

analyte chemical class(es)

-

analyte chemical class(es)

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

20-25°C

storage temp.

2-8°C

General description

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer.

Application

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4. It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Linkage

Suspension of Product No. G 4510

Physical form

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

180.1 °F

flash_point_c

82.3 °C


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L-Glutathione reduced ≥98.0%

Sigma-Aldrich

G4251

L-Glutathione reduced

F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
Paul R Jenkins et al.
Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)
We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
Diane M Kanter et al.
Nucleic acids research, 39(7), 2580-2592 (2010-11-27)
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2
Carine Aubry et al.
Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
Xiu Feng Hu et al.
The Journal of clinical investigation, 119(2), 362-375 (2009-01-17)
Provirus integration site for Moloney murine leukemia virus (PIM1) is a proto-oncogene that encodes a serine/threonine kinase with multiple cellular functions. Overexpression of PIM-1 plays a critical role in progression of prostatic and hematopoietic malignancies. Here we describe the generation

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