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GE28-4017-47

GSTrap 4B

Cytiva 28-4017-47, pack of 1 × 5 mL

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NACRES:
NA.56

ligand

glutathione

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 28-4017-47

parameter

<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi (H2O at 25°C)

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

General description

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

Application

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

Features and Benefits

  • High binding capacity.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
  • GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

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Anh Hoang Le et al.
The Journal of cell biology, 220(9) (2021-06-25)
The Scar/WAVE complex drives actin nucleation during cell migration. Interestingly, the same complex is important in forming membrane ruffles during macropinocytosis, a process mediating nutrient uptake and membrane receptor trafficking. Mammalian CYRI-B is a recently described negative regulator of the

Articles

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

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This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

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This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

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This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

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Protocols

Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B Columns

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service