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GE17-5131-02

GSTrap Fast Flow

Cytiva 17-5131-02, pack of 5 × 5 mL

NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5131-02

parameter

flow rate
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 4% agarose

average diameter

90 μm

cleaning

3-12

working range

3-12

capacity

>10 mg/mL binding capacity(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

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GSTrap&#8482; High Performance Cytiva 17-5282-02, pack of 5 × 5&#160;mL

GE17-5282-02

GSTrap High Performance

ligand

glutathione and 10-carbon linker arm

ligand

glutathione and 10-carbon linker arm

ligand

glutathione

ligand

glutathione and 10-carbon linker arm

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 17-5131-02

manufacturer/tradename

Cytiva 17-5130-01

manufacturer/tradename

Cytiva 17-5282-02

manufacturer/tradename

Cytiva 17-5132-02

parameter

flow rate

parameter

flow rate, 42 psi

parameter

<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi (H2O at room temperature.)

parameter

-

bed size

16 mm × 25 mm

bed size

7 mm × 25 mm

bed size

16 mm × 25 mm

bed size

-

General description

GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC System.

* See the section for licensing information in our terms and conditions.

Features and Benefits

  • Fast and simple one-step purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy scale-up by connecting the columns in series.
  • Sepharose 4 Fast Flow provides good flow properties.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


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Articles

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

Protocols

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Performing a Separation with Cytiva Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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