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ligand
glutathione and 10-carbon linker arm
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5131-02
parameter
flow rate
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning
3-12
working range
3-12
capacity
>10 mg/mL binding capacity(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
Related Categories
1 of 4
This Item | GE17-5130-01 | GE17-5282-02 | GE17-5132-02 |
|---|---|---|---|
| ligand glutathione and 10-carbon linker arm | ligand glutathione and 10-carbon linker arm | ligand glutathione | ligand glutathione and 10-carbon linker arm |
| shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. | shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| manufacturer/tradename Cytiva 17-5131-02 | manufacturer/tradename Cytiva 17-5130-01 | manufacturer/tradename Cytiva 17-5282-02 | manufacturer/tradename Cytiva 17-5132-02 |
| parameter flow rate | parameter flow rate, 42 psi | parameter <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi (H2O at room temperature.) | parameter - |
| bed size 16 mm × 25 mm | bed size 7 mm × 25 mm | bed size 16 mm × 25 mm | bed size - |
General description
GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.
* See the section for licensing information in our terms and conditions.
Features and Benefits
- Fast and simple one-step purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Easy scale-up by connecting the columns in series.
- Sepharose™ 4 Fast Flow provides good flow properties.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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Customers Also Viewed
Articles
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Protocols
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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