GE28-4055-00

GST MultiTrap^™ 4B

Cytiva 28-4055-00

• UNSPSC Code: 12161501
• NACRES: NA.32

Properties

• ligand: glutathione
• material: polypropylene and polyethylene
• manufacturer/tradename: Cytiva 28-4055-00
• packaging: pkg of 4 x 96-well
• size: 127.8 mm × 85.5 mm × 30.6 mm
• matrix: 4% agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 4-13
• working range: 4-13
• capacity: >25 mg binding capacity (horse liver GST/ml medium)

Description

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

GST MultiTrap^™ FF and GST MultiTrap^™ 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

Application

GST MultiTrap^™ FF and GST MultiTrap^™ 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap^™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap^™ FF is prepacked with Glutathione Sepharose^™ 4 Fast Flow, while GST MultiTrap^™ 4B is prepacked with Glutathione Sepharose^™ 4B.

Features and Benefits

• 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
• Highly reproducible well-to well and plate-to-plate results.
• High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
• Wells are prepacked with Glutathione Sepharose^™ media for convenience.
• Easy and predictable scale-up using prepacked GSTrap^™ FF, GSTPrep^™ FF 16/10, and GSTrap^™ 4B columns.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GSTPrep is a trademark of Cytiva

GSTrap is a trademark of Cytiva

MultiTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids