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GE28-4009-89

His MultiTrap High Performance

Cytiva 28-4009-89, pack of 4 plates

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NACRES:
NA.32

material

polypropylene and polyethylene

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 4 plates

manufacturer/tradename

Cytiva 28-4009-89

size

127.8 mm × 85.5 mm × 30.6 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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GE28-4009-90GE17-0920-03GE17-0920-05
vibrant-m

GE28-4009-90

His MultiTrap Fast Flow

size

127.8 mm × 85.5 mm × 30.6 mm

size

127.8 mm × 85.5 mm × 30.6 mm

size

-

size

-

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 4 plates

packaging

pack of 4 plates

packaging

pack of 5 × 1 mL

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 28-4009-89

manufacturer/tradename

Cytiva 28-4009-90

manufacturer/tradename

Cytiva 17-0920-03

manufacturer/tradename

Cytiva 17-0920-05

matrix

highly cross-linked 6% agarose

matrix

6% cross-linked agarose

matrix

highly cross-linked 6% agarose

matrix

highly cross-linked 6% agarose

General description

His MultiTrap FF and His MultiTrap HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).

Application

His MultiTrap FF and His MultiTrap HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose 6 Fast FLow (His MultiTrap FF) or Ni Sepharose High Performance (His MultiTrap HP). A protocol for His MultiTrap is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.

Loading unclarified samples directly to His MultiTrap FF and His MultiTrap HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap FF and His MultiTrap HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

Features and Benefits

  • Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
  • Increased convenience and consistency with prepacked 96-well plates.
  • No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
  • Use His MultiTrap 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
  • Easy scale-up to HisTrap FF, HisPrep FF 16/10 or HisTrap HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Environment

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

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Articles

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.

How to optimize purification of histidine-tagged proteins using Cytiva products.

See All

Protocols

This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from Cytiva.

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

This page shows how to purify protein A-tagged proteins using Cytiva products.

How to isolate proteins from inclusion bodies using Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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