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Key Documents

GE28-9180-08

PD Miditrap G-25

Cytiva 28-9180-08

Synonym(s):

G-25 Miditrap, Miditrap G-25, PD G-25 Miditrap

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About This Item

UNSPSC Code:
41115709
NACRES:
NA.56

feature

wetted part: no

packaging

pkg of 50 ea (columns)

manufacturer/tradename

Cytiva 28-9180-08

storage condition

dry media

bed size

13 mm × 26 mm

bed volume

3.5 mL

column I.D.

13 mm

matrix

cross-linked dextran

particle size

>50 μm (dry)
85-260 μm

cleaning

2-13

working range

2-13

suitability

suitable for bioprocess medium

Related Categories

General description

PD MidiTrap G-25 is a convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel. Can be used in a centrifugation or gravity protocol.

PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.

Application

Protein sample preparation. Desalting/Buffer Exchange/Cleanup.

Features and Benefits

  • Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
  • Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
  • High desalting capacity.
  • Flexible protocols with ability to use gravity flow or centrifugation.
  • Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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