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MilliporeSigma

GE28-9180-04

PD Spintrap™ G-25

Cytiva 28-9180-04

Synonym(s):

G-25 Spin Column

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500
Packaging:
pkg of 1 ea

Key Documents

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Technical Service
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Product Name

PD Spintrap™ G-25, Cytiva 28-9180-04

feature

wetted part: no

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9180-04

storage condition

dry media

matrix

cross-linked dextran

particle size

>50 μm (dry)
85-260 μm

cleaning

2-13

working range

2-13

suitability

suitable for bioprocess medium

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Preparation Note

Store at 4 to 30 °C (0,05% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

SpinTrap is a trademark of Cytiva

Application

Protein sample preparation. Desalting/Buffer Exchange/Cleanup.

Features and Benefits

  • Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with the possibility to run multiple samples in parallel.
  • Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
  • High desalting capacity.
  • Reproducible, high throughput, parallel cleanup of protein samples.
  • Sample preparation in the range of 100 to 180 μl without sample dilution.

General description

PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules. PD SpinTrap G-25 is a single-use microspin column that is designed for desalting and buffer exchange of biological sample using a standard microcentrifuge. The column is prepacked with ~600 µL Sephadex G-25 resin, and the column material is biocompatible polypropylene. Instructions supplied with the columns.

Storage Class

12 - Non Combustible Liquids

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Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Protocols

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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