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GE28-4083-47

Ab Spintrap

Cytiva 28-4083-47

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NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 50 × 100 μL

manufacturer/tradename

Cytiva 28-4083-47

storage condition

(20% Ethanol)

bed volume

100 μL

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9

capacity

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(IgG/column)

storage temp.

2-8°C

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Ab Spintrap

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packaging

pkg of 50 × 100 μL

packaging

pack of 2 × 1 mL

packaging

pkg of 1 ea

packaging

pack of 1 mL

ligand

recombinant protein G lacking albumin-binding region

ligand

native protein A

ligand

-

ligand

quaternary amine

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 28-4083-47

manufacturer/tradename

Cytiva 17-0402-03

manufacturer/tradename

Cytiva 28-9180-04

manufacturer/tradename

Cytiva 29-0513-25

storage condition

(20% Ethanol)

storage condition

(20% Ethanol)

storage condition

dry media

storage condition

-

General description

Ab SpinTrap are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.

Application

Ab SpinTrap is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap contains Protein G Sepharose High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap together with buffers prepared using Ab Buffer Kit.

Features and Benefits

  • Rapid and convenient small-scale purification of IgG.
  • Protein G Sepharose High Performance in prepacked spin columns.
  • No filtration or centrifugation of different crude samples necessary for antibody purification.
  • Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Articles

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service