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GE28-9852-55

Protein G GraviTrap

Cytiva 28-9852-55, pack of 10 × 1 mL

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NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 10 × 1 mL

manufacturer/tradename

Cytiva 28-9852-55

storage condition

(20% Ethanol)

bed volume

1 mL

capacity

~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

storage temp.

2-8°C

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Protein G GraviTrap™ Cytiva 28-9852-55, pack of 10 × 1 mL

GE28-9852-55

Protein G GraviTrap

GST GraviTrap™ Cytiva 28-9523-60

GE28-9523-60

GST GraviTrap

rProtein A GraviTrap™ Cytiva 28-9852-54, pack of 10 × 1 mL

GE28-9852-54

rProtein A GraviTrap

His GraviTrap™ Cytiva 11-0033-99

GE11-0033-99

His GraviTrap

packaging

pack of 10 × 1 mL

packaging

pkg of 10 ea

packaging

pack of 10 × 1 mL

packaging

pkg of 10 ea

storage condition

(20% Ethanol)

storage condition

-

storage condition

(20% Ethanol)

storage condition

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

-

manufacturer/tradename

Cytiva 28-9852-55

manufacturer/tradename

Cytiva 28-9523-60

manufacturer/tradename

Cytiva 28-9852-54

manufacturer/tradename

Cytiva 11-0033-99

bed volume

1 mL

bed volume

2 mL

bed volume

1 mL

bed volume

1 mL

General description

Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 mL of Protein G Sepharose 4 Fast Flow.

Protein G GraviTrap are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Application

Fast and efficient manual purification of monoclonal and polyclonal antibodies

Features and Benefits

  • Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
  • High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as Protein G GraviTrap ~ 20 mg human IgG/column

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

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25G
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705578-5MG-PW

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Articles

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service