MilliporeSigma
All Photos(1)

Documents

GE28-9852-56

rProtein A/Protein G GraviTrap

Cytiva 28-9852-56, pack of 10 × 1 mL

Sign Into View Organizational & Contract Pricing

NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 10 × 1 mL

manufacturer/tradename

Cytiva 28-9852-56

storage condition

(20% Ethanol)

bed volume

1 mL

capacity

35 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

storage temp.

2-8°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
GE28-9852-54GE28-9523-60GE28-9852-55
rProtein A GraviTrap™ Cytiva 28-9852-54, pack of 10 × 1 mL

GE28-9852-54

rProtein A GraviTrap

GST GraviTrap™ Cytiva 28-9523-60

GE28-9523-60

GST GraviTrap

Protein G GraviTrap™ Cytiva 28-9852-55, pack of 10 × 1 mL

GE28-9852-55

Protein G GraviTrap

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 28-9852-56

manufacturer/tradename

Cytiva 28-9852-54

manufacturer/tradename

Cytiva 28-9523-60

manufacturer/tradename

Cytiva 28-9852-55

storage condition

(20% Ethanol)

storage condition

(20% Ethanol)

storage condition

-

storage condition

(20% Ethanol)

bed volume

1 mL

bed volume

1 mL

bed volume

2 mL

bed volume

1 mL

capacity

35 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

capacity

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

capacity

≥20 mg binding capacity(recombinant glutathione S-transferase (rGST)), >25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)

capacity

~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

General description

rProtein A Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 mL of a mixture of 50% rProtein A Sepharose Fast FLow and 50% protein G Sepharose 4 Fast Flow.

Application

rProtein A Protein G GraviTrap are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A and Protein G ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Features and Benefits

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as Protein A/Protein G GraviTrap ~ 35 mg human IgG/column.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Articles

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service