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GE28-9031-34

Protein G High Performance Spintrap

Cytiva 28-9031-34, pack of 16 columns

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NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

wetted part: no

packaging

pack of 16 columns

manufacturer/tradename

Cytiva 28-9031-34

storage condition

(20% Ehtanol)
(20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

capacity

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(human IgG/column)

storage temp.

2-8°C

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This Item
GE28-9031-32GE17-0404-01GE17-0404-03
packaging

pack of 16 columns

packaging

pack of 16 columns

packaging

pack of 5 × 1 mL

packaging

pack of 2 × 1 mL

matrix

highly cross-linked 6% agarose

matrix

highly cross-linked 6% agarose

matrix

-

matrix

-

ligand

recombinant protein G lacking albumin-binding region

ligand

native protein A

ligand

recombinant protein G lacking albumin-binding region

ligand

recombinant protein G lacking albumin-binding region

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 28-9031-34

manufacturer/tradename

Cytiva 28-9031-32

manufacturer/tradename

Cytiva 17-0404-01

manufacturer/tradename

Cytiva 17-0404-03

General description

protein G HP SpinTrap is a prepacked, single-use spin column containing protein G Sepharose High Performance.

Application

Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Features and Benefits

  • For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Articles

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service