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DFF100

Sigma-Aldrich

DEAE–Sepharose

Fast Flow

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Synonym(s):
Diethylaminoethyl–Sepharose
CAS Number:
MDL number:
NACRES:
NA.56

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

capacity

110-160 μeq/mL binding capacity(gel volume)

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1 of 4

This Item
DCL6B100A50120CCF100
vibrant-m

DFF100

DEAE–Sepharose

vibrant-m

DCL6B100

DEAE–Sepharose

vibrant-m

A50120

DEAE–Sephadex®

vibrant-m

CCF100

CM Sepharose

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

capacity

110-160 μeq/mL binding capacity(gel volume)

capacity

130-170 μeq/mL binding capacity (gel volume)(gel volume)

capacity

3-4 meq/g binding capacity

capacity

90-130 μeq/mL(gel basis)

bead size

45-165 μm (wet)

bead size

45-165 μm

bead size

40-125 μm (dry)

bead size

-

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose has been used in:
  • anion exchange chromatography
  • to screen polyisoprenyl phosphate phosphatase activity
  • to purify isoinhibitors
  • for the purification of human immunodeficiency virus (HIV)
  • glycoprotein envelope (gp140 env)

DEAE-Sepharose is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

replaced by

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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G L Yewey et al.
Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)
Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal
Advances in Potato Chemistry and Technology (2016)
T M Schmidt et al.
Applied and environmental microbiology, 55(10), 2607-2612 (1989-10-01)
Luminescence of batch cultures of Xenorhabdus luminescens was maximal when cultures approached stationary phase; the onset of in vivo luminescence coincided with a burst of synthesis of bacterial luciferase, the enzyme responsible for luminescence. Expression of luciferase was aldehyde limited
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