MilliporeSigma
All Photos(1)

Documents

GE17-0709-10

DEAE Sepharose Fast Flow

Cytiva 17-0709-10, pack of 25 mL

Sign Into View Organizational & Contract Pricing

NACRES:
NA.56

ligand

diethylaminoethyl

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-0709-10

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

2-12

suitability

suitable for bioprocess medium

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
GE17-0729-10GE17-0709-01GE17-0510-10
DEAE Sepharose™ Fast Flow Cytiva 17-0709-10, pack of 25 mL

GE17-0709-10

DEAE Sepharose Fast Flow

SP Sepharose™ Fast Flow Cytiva 17-0729-10, pack of 25 mL

GE17-0729-10

SP Sepharose Fast Flow

DEAE Sepharose™ Fast Flow Cytiva 17-0709-01, pack of 500 mL

GE17-0709-01

DEAE Sepharose Fast Flow

Q Sepharose™ Fast Flow Cytiva 17-0510-10, pack of 25 mL

GE17-0510-10

Q Sepharose Fast Flow

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

manufacturer/tradename

Cytiva 17-0709-10

manufacturer/tradename

Cytiva 17-0729-10

manufacturer/tradename

Cytiva 17-0709-01

manufacturer/tradename

Cytiva 17-0510-10

packaging

pack of 25 mL

packaging

pack of 25 mL

packaging

pack of 500 mL

packaging

pack of 25 mL

ligand

diethylaminoethyl

ligand

sulphopropyl

ligand

diethylaminoethyl

ligand

quaternary amine

description

Ion Exchanger Type (value)

description

Ion Exchanger Type (value)

description

Ion Exchanger Type (value)

description

Ion Exchanger Type (value)

General description

DEAE Sepharose Fast FLow is a weak anion exchanger based on the well established Sepharose Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Application

DEAE Sepharose Fast FLow is part of the Sepharose Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. DEAE Sepharose Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with DEAE Sepharose Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, DEAE Sepharose Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Well-proven weak anion exchanger developed for industrial downstream processes
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 10

1 of 10

DEAE–Sephadex®

Sigma-Aldrich

A50120

DEAE–Sephadex®

Capto™ DEAE Cytiva 17-5443-01, pack of 100 mL

GE17-5443-01

Capto DEAE

SP Sepharose™ Fast Flow Cytiva 17-0729-10, pack of 25 mL

GE17-0729-10

SP Sepharose Fast Flow

CM Sepharose™ Fast Flow Cytiva 17-0719-01, pack of 500 mL

GE17-0719-01

CM Sepharose Fast Flow

SP Sepharose™ Fast Flow Cytiva 17-0729-01, pack of 300 mL

GE17-0729-01

SP Sepharose Fast Flow

DEAE-Sephacel Cytiva 17-0500-01, pack of 500 mL

GE17-0500-01

DEAE-Sephacel

CM Sepharose™ Fast Flow

Sigma-Aldrich

CCF100

CM Sepharose

HiPrep™ CM Fast Flow 16/10 Cytiva 28-9365-42

GE28-9365-42

HiPrep CM Fast Flow 16/10

Articles

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service