GE17-0405-01

HiTrap^® Protein G High Performance

Cytiva 17-0405-01, pack of 1 × 5 mL

• Synonym(s): Protein G affinity column, Protein purification column
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: recombinant protein G lacking albumin-binding region
• packaging: pack of 1 × 5 mL
• manufacturer/tradename: Cytiva 17-0405-01
• storage condition: (20% Ehtanol); (20% Ethanol)
• parameter: flow rate; 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-9
• working range: 3-9
• capacity: >1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.); 125 mg binding capacity(human IgG/column)
• storage temp.: 2-8°C

Description

General description

HiTrap^® Protein G HP columns are prepacked with Protein G Sepharose^™ High Performance for fast, easy separations of IgG from different species and subclasses.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

• High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
• 34 μm bead size for high performance, high-resolution purifications.
• Binds broad range of different species and subclasses of IgG.
• Simple and proven method giving reproducible results.
• Designed for use with syringe, peristalic pump, or chromatography system.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids