MilliporeSigma
All Photos(1)

Documents

GE28-4013-53

His Spintrap

Cytiva 28-4013-53

Sign Into View Organizational & Contract Pricing

NACRES:
NA.32

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 28-4013-53

packaging

pkg of 50 x 100 μL

bed volume

100 μL

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
GE28-4083-47GE11-0033-99GE28-9031-34
His Spintrap™ Cytiva 28-4013-53

GE28-4013-53

His Spintrap

Ab Spintrap™ Cytiva 28-4083-47

GE28-4083-47

Ab Spintrap

His GraviTrap™ Cytiva 11-0033-99

GE11-0033-99

His GraviTrap

packaging

pkg of 50 x 100 μL

packaging

pkg of 50 × 100 μL

packaging

pkg of 10 ea

packaging

pack of 16 columns

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 28-4013-53

manufacturer/tradename

Cytiva 28-4083-47

manufacturer/tradename

Cytiva 11-0033-99

manufacturer/tradename

Cytiva 28-9031-34

bed volume

100 μL

bed volume

100 μL

bed volume

1 mL

bed volume

-

avg. part. size

34 μm

avg. part. size

34 μm

avg. part. size

-

avg. part. size

34 μm

General description

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)

* See the section for licensing information in our terms and conditions.

Purification of unclarified samples on His SpinTrap columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.

His SpinTrap columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap together with buffers prepared using His Buffer Kit.

The columns are prepacked with Ni Sepharose High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose High Performance medium is negligible.

Features and Benefits

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 μg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

Storage and Stability

Store at 4 to 30 °C (0.15% Kathon CG)
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

pictograms

Environment

signalword

Warning

hcodes

Storage Class

12 - Non Combustible Liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Articles

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protocols

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service