GE28-9356-07

HiPrep^™ 26/60 Sephacryl^® S-500 HR

Cytiva 28-9356-07, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

• Synonym(s): Sephacryl S-500 HR
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• material: polypropylene (PP)
• mol wt: 40000-20000000 Da (globular proteins)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9356-07
• parameter: <1.3 mL/min flow rate; <13 mL sample volume; 0.15 Mpa (22 psi)
• bed volume: 320 mL
• column L × I.D.: 60 cm × 26 mm
• matrix: dextran-acrylamide particle platform; allyl dextran and N, N’-methylenebisacrylamide; polymeric
• matrix active group: dextran-acrylamide phase
• avg. part. size: 50 μm
• cleaning: 2-13
• working range: 3-11
• separation technique: size exclusion (SEC)

Description

General description

HiPrep 26/60 Sephacryl S-500 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl S-500 is used for separating polysaccarides, macromolecules with extended structures, and even small plasmids.

Features and Benefits

• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
• Gel filtration columns for low to medium pressure ÄKTA systems.
• Well-established in industrial production.
• The HiPrep Sephacryl series offers reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep Sephacryl columns speed up protein purification.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

gel filtration columns for low to medium pressure ÄKTA systems+AE14

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva

Sephacryl is a registered trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids