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GE28-9909-44

Sigma-Aldrich

Superdex® 200 Increase 10/300 GL

Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size

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NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superdex®

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

10000-600000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9909-44

parameter

<1.8 mL/min flow rate
<1.80 mL/min flow rate
25-500 μL sample volume
3.0 Mpa (580 psi)

bed volume

~24 mL

column L × I.D.

30 cm × 10 mm

matrix

agarose-dextran composite
polymeric

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

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This Item
GE28-9909-45GE29321905GE29219757
Superdex&#174; 30 Increase 10/300 GL, Cytiva

GE29219757

Superdex® 30 Increase

product line

Superdex®

product line

Superdex®

product line

-

product line

-

packaging

pkg of 1 ea

packaging

1 ea of

packaging

-

packaging

-

matrix

agarose-dextran composite, polymeric

matrix

agarose dextran composite, polymeric

matrix

-

matrix

-

particle size

8.6 μm

particle size

8.6 μm

particle size

-

particle size

-

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

-

separation technique

-

General description

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification and also for characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as aggregate analysis of antibodies, size-homegeneity analysis of membrane proteins, or protein-protein interactions. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Suitability

For both preparative and analytical purposes
Gel filtration for small-scale (25-500 mL) preparative purification.
Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


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Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Setting Column Pressure Limits for Size Exclusion Chromatography

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Refolding of proteins using gel filtration

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Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

HIC Purification Options and Scale up with SOURCE™ Media

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Related Content

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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