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GE29-0915-98

Sigma-Aldrich

Superose® 6 Increase

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

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NACRES:
SB.52

material

glass column

product line

Superose®

mol wt

5000-5000000 Da (globular proteins)

manufacturer/tradename

Cytiva 29-0915-98

parameter

<0.03 mL/min flow rate (20% ethanol at low temp)
<0.07 mL/min flow rate (20% ethanol at room temp)
<0.07 mL/min flow rate (water at low temp)
<0.15 mL/min flow rate (water at room temp)
3 Mpa (435 psi)
4-50 μL sample volume

matrix

cross-linked agarose
polymeric

matrix active group

agarose phase

avg. part. size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

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GE29323952GE29-1487-22GE29-1487-21
vibrant-m

GE29-0915-98

Superose® 6 Increase

vibrant-m

GE29-1487-22

Superdex® Increase 75

vibrant-m

GE29-1487-21

Superdex® Increase 75

separation technique

size exclusion (SEC)

separation technique

-

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

matrix

cross-linked agarose, polymeric

matrix

-

matrix

polymeric

matrix

polymeric

material

glass column

material

-

material

glass column, titanium frit

material

glass column, titanium frit

product line

Superose®

product line

-

product line

Superdex®

product line

Superdex®

mol wt

5000-5000000 Da (globular proteins)

mol wt

-

mol wt

3000-70,000 Da (globular proteins)

mol wt

3000-70,000 Da (globular proteins)

General description

Superose® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

Features and Benefits

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Storage Class

3 - Flammable liquids


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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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Customers Also Viewed

Articles

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocols

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Related Content

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service