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S500HR

Sigma-Aldrich

Sephacryl®

500-HR, MW range 40-20,000 kDa (dextrans)

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Synonym(s):
Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
CAS Number:
MDL number:

Quality Level

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

40-20,000 kDa MW range (dextrans)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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1 of 4

This Item
S400HRS200HRS300HR
Sephacryl® 500-HR, MW range 40-20,000 kDa (dextrans)

S500HR

Sephacryl®

Sephacryl® 400-HR, MW range 20-8000 kDa (globular proteins)

S400HR

Sephacryl®

Sephacryl® 200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

S200HR

Sephacryl®

Sigma-Aldrich

S300HR

Sephacryl®

particle size

25-75 μm (wet)

particle size

25-75 μm (wet)

particle size

25-75 μm (wet)

particle size

25-75 μm (wet)

pore size

40-20,000 kDa MW range (dextrans)

pore size

10-2000 kDa MW range (dextrans), 20-8000 kDa MW range (globular proteins)

pore size

1-80 kDa MW range (dextrans), 5-250 kDa MW range (globular proteins)

pore size

1-400 kDa MW range (dextrans), 10-1500 kDa MW range (globular proteins)

form

suspension (20% aqueous ethanol)

form

suspension (20% aqueous ethanol)

form

suspension (20% aqueous ethanol)

form

suspension (20% aqueous ethanol)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

feature

autoclavable

feature

autoclavable

feature

autoclavable

feature

autoclavable

General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S500HR-750ML′s updated product number is GE17-0613-01

Application

Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum, dextran-RBA12 from Weissella cibaria and dextran from Weissella cibaria JAG8.
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

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Theresa L Gioannini et al.
The Journal of biological chemistry, 277(49), 47818-47825 (2002-10-10)
Experiments utilizing endotoxin aggregates, lipooligosaccharides (LOS) isolated from metabolically labeled Neisseria meningitidis serotype group B, demonstrate that albumin is an essential component of lipopolysaccharide binding protein- (LBP) and sCD14-dependent 1) disaggregation of LOS and 2) LOS activation of human umbilical
M A Karymov et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(14), 2631-2641 (2001-12-01)
Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin
Functional food applications of dextran from Weissella cibaria RBA12 from pummelo (Citrus maxima)
Baruah R, et al.
International Journal of Food Microbiology, 242, 124-131 (2017)
New structural features of Acacia tortuosa gum exudate
Martinez M, et al.
Food Chemistry, 182, 105-110 (2015)
Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive
Tingirikari JM, et al.
International Journal of Food Sciences and Nutrition, 65(6), 686-691 (2014)

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