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S200HR

Sigma-Aldrich

Sephacryl®

200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

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Synonym(s):
Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
CAS Number:
MDL number:
NACRES:
NA.56

Quality Level

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

1-80 kDa MW range (dextrans)
5-250 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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Show Differences

1 of 4

This Item
S300HRS400HRCCF100
vibrant-m

S200HR

Sephacryl®

vibrant-m

S300HR

Sephacryl®

vibrant-m

S400HR

Sephacryl®

vibrant-m

CCF100

CM Sepharose

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

matrix

-

pore size

1-80 kDa MW range (dextrans), 5-250 kDa MW range (globular proteins)

pore size

1-400 kDa MW range (dextrans), 10-1500 kDa MW range (globular proteins)

pore size

10-2000 kDa MW range (dextrans), 20-8000 kDa MW range (globular proteins)

pore size

4,000,000 exclusion limit (av. mol. wt.)

form

suspension (20% aqueous ethanol)

form

suspension (20% aqueous ethanol)

form

suspension (20% aqueous ethanol)

form

suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S200HR-750ML′s updated product number is GE17-0584-01

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

Related product

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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