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GE28-9909-45

Sigma-Aldrich

Superdex® 200 Increase 5/150 GL

Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size

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NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superdex®

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

10000-600000 Da (globular proteins)

manufacturer/tradename

Cytiva 28-9909-45

packaging

1 ea of

parameter

<0.75 mL/min flow rate
<0.75 ml/min flow rate
<3.0 Mpa (725 psi)
4-50 μL sample volume

bed volume

~3 mL

column L × I.D.

15 cm × 5 mm

matrix

agarose dextran composite
polymeric

matrix active group

phenyl phase

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

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1 of 4

This Item
GE28-9909-44GE28-9909-46GE17-1043-01
Superdex&#174; 200 Prep Grade Cytiva 17-1043-01, pack of 150&#160;mL

GE17-1043-01

Superdex® 200 Prep Grade

product line

Superdex®

product line

Superdex®

product line

Superdex®

product line

-

packaging

1 ea of

packaging

pkg of 1 ea

packaging

1 ea of

packaging

pack of 150 mL

matrix

agarose dextran composite, polymeric

matrix

agarose-dextran composite, polymeric

matrix

agarose-dextran composite, polymeric

matrix

composite of cross-linked agarose and dextran

matrix active group

phenyl phase

matrix active group

-

matrix active group

-

matrix active group

-

particle size

8.6 μm

particle size

8.6 μm

particle size

8.6 μm

particle size

24-44 μm

General description

Superdex 200 is a column for rapid purity check and screening by high resolution gel filtration for proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Suitability

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
Well suited for rapid purity checks and quick screenings of experimental conditions.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Articles

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

This page shows how to perform a purification of His-tagged membrane proteins.

Protocols

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

This page shows how to solubilize membrane proteins with products from Cytiva.

Related Content

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service