GE28-9924-73

HiScreen^™ Capto^™ Butyl

Cytiva 28-9924-73, pack of 1 column

• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• ligand: butyl
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• packaging: pack of 1 column
• manufacturer/tradename: Cytiva 28-9924-73
• bed size: 7.7 mm × 100 mm
• column I.D.: 7.7 mm
• matrix: highly cross-linked agarose
• avg. part. size: 75 μm (d50v)
• cleaning: 3-14
• working range: 3-13
• capacity: 27 mg binding capacity(27 mg BSA /ml (at QB10%))
• suitability: suitable for bioprocess medium

Description

General description

Capto^™ Phenyl (High sub) and Capto^™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.

Application

HiScreen^™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen^™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen^™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

Capto^™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto^™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose^™ 4 Fast Flow. Higher flow rates can be used with Capto^™ Butyl during equilibration, wash and regeneration.

Features and Benefits

• Prepacked with Capto^™ Butyl
• Excellent for method optimization and parameter screening due to the 10 cm bed height
• Easily connected in series to achieve 20 cm bed height
• Small bed volume for fast results and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501