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H5916

Sigma-Aldrich

Human Growth Hormone human

HGH, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym(s):

HGH

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

100

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤0.5 ng/mL EC50

mol wt

monomer 22 kDa (non-glycosylated)

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mg

storage temp.

−20°C

General description

Human growth hormone (HGH), also called somatotropin, is a peptide hormone that consists of a long single chain of amino acids. It is naturally secreted by somatotropic cells in the anterior pituitary gland of the human body.

Application

Human Growth Hormone human has been used as a supplement in culture media to study its effect on the immune response in fish cell lines. It has also been used as a supplement in biliary differentiation medium (BDM) for differentiation of human embryonic stem cells (hESCs)/ human induced pluripotent stem cells (hiPSCs)-derived hepatoblasts.

Biochem/physiol Actions

Human growth hormone (HGH) is required for normal human growth and development. HGH modifies a variety of physiological functions by stimulating the expression of insulin-like growth factor I and increases calcium retention and bone density. HGH increases muscle mass, promotes lipolysis, and reduces liver uptake of glucose. Abnormalities in the regulation of HGH lead to various growth disorders such as gigantism or acromegaly due to hypersecretion of HGH. Conversely, HGH deficiency leads to growth deficit in children and GH deficiency syndrome in adults.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine, Human Growth Hormone (HGH) is expressed as a non-glycosylated monomer in human HEK 293 cells and has an apparent molecular mass of 22 kDa.

Analysis Note

The activity was determined by the dose-dependent stimulation of the proliferation of rat lymphoma line Nb2-11 cells (prolactin indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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J P Pontigo et al.
General and comparative endocrinology, 300, 113631-113631 (2020-10-04)
To clarify the role of growth hormone (GH) in the immune system of fish, we examine the comparative effect of GH and Growth Hormone Release Factor (GRF) on leukocytes culture of the head kidney of Atlantic salmon and the SHK-1
Wen-Jen Chung et al.
Protein expression and purification, 110, 79-88 (2015-02-24)
Growth hormone (GH) performs important roles in regulating somatic growth, reproduction, osmoregulation, metabolism and immunity in teleosts, and thus, it has attracted substantial attention in the field of aquaculture application. Herein, giant grouper GH (ggGH) cDNA was cloned into the
Maximilian Bielohuby et al.
Disease models & mechanisms, 7(11), 1263-1273 (2014-09-23)
The development of new growth hormone (GH) agonists and growth hormone antagonists (GHAs) requires animal models for pre-clinical testing. Ideally, the effects of treatment are monitored using the same pharmacodynamic marker that is later used in clinical practice. However, intact
Maïté Verreault et al.
Clinical and translational medicine, 12(7), e939-e939 (2022-07-10)
New therapeutic approaches are needed to improve the prognosis of glioblastoma (GBM) patients. With the objective of identifying alternative oncogenic mechanisms to abnormally activated epidermal growth factor receptor (EGFR) signalling, one of the most common oncogenic mechanisms in GBM, we
Noushin Dianat et al.
Hepatology (Baltimore, Md.), 60(2), 700-714 (2014-04-10)
Cholangiocytes are biliary epithelial cells, which, like hepatocytes, originate from hepatoblasts during embryonic development. In this study we investigated the potential of human embryonic stem cells (hESCs) to differentiate into cholangiocytes and we report a new approach, which drives differentiation

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Chromatograms

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