Skip to Content
Merck
All Photos(1)

Key Documents

H7787

Millipore

HIS-Select® Spin Columns

Synonym(s):

His-Tag Spin Columns

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Pricing and availability is not currently available.

shelf life

2 yr at 2‑8 °C

Quality Level

packaging

pkg of 10 ea
pkg of 50 ea

pore size

100 nm pore size

capacity

>0.5 mg/column

storage temp.

2-8°C

General description

Kit contains 3 collection tubes for every spin column.

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

  • High Selectivity for higher purity
  • Convenient and Ready-to-Use
  • Consistent protein binding each use
  • Compatible with CelLytic cell lysis reagents

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Maria A Schumacher et al.
Nucleic acids research, 46(14), 7405-7417 (2018-06-16)
Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
Mutational analysis of the cleavage of the cancer-associated laminin receptor by stromelysin-3 reveals the contribution of flanking sequences to site recognition and cleavage efficiency
Fiorentino,M et al.
International Journal of Molecular Medicine, 23(3), 387-397 (2009)
Wenbo Li et al.
Drug design, development and therapy, 10, 3415-3423 (2016-11-02)
Choroidal neovascularization (CNV) in age-related macular degeneration usually causes blindness. We established a novel targeted inhibitor for CNV in age-related macular degeneration. The inhibitor CR2-sFlt 1 comprises a CR2-targeting fragment and an anti-vascular endothelial growth factor (VEGF) domain (sFlt 1).
Maureen J Bibb et al.
Molecular microbiology, 84(6), 1033-1049 (2012-05-16)
The chaplin and rodlin proteins together constitute the major components of the hydrophobic sheath that coats the aerial hyphae and spores in Streptomyces, and mutants lacking the chaplins are unable to erect aerial hyphae and differentiate on minimal media. We
Ozan Gundogdu et al.
Journal of bacteriology, 193(16), 4238-4249 (2011-06-07)
Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment and so must possess regulatory systems to sense and adapt to external stimuli, such as oxidative

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service