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I3909

Sigma-Aldrich

Ionomycin calcium salt

Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO

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Empirical Formula (Hill Notation):
C41H70CaO9
Molecular Weight:
747.07
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.85

biological source

Streptomyces conglobatus

Quality Level

assay

≥98% (HPLC)

shelf life

2 yr

storage condition

(Tightly closed. )

concentration

1 mM in DMSO

color

colorless

mp

18.4 °C

solubility

DMSO: 1 mM

antibiotic activity spectrum

Gram-positive bacteria

mode of action

cell membrane | interferes

shipped in

wet ice

storage temp.

−20°C

SMILES string

C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O

InChI

1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

InChI key

WKRWUYKLUMMAKG-WYGBAUISSA-L

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This Item
I9657I0634SBR00001
Ionomycin calcium salt Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO

I3909

Ionomycin calcium salt

Vancomycin hydrochloride Ready Made Solution, 100 mg/mL in DMSO

SBR00001

Vancomycin hydrochloride

mode of action

cell membrane | interferes

mode of action

cell membrane | interferes

mode of action

cell membrane | interferes

mode of action

cell wall synthesis | interferes

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

-

biological source

Streptomyces conglobatus

biological source

Streptomyces conglobatus

biological source

-

biological source

Streptomyces orientalis

solubility

DMSO: 1 mM

solubility

acetonitrile: 10 mg/mL

solubility

DMSO: 10 mg/mL, ethanol: soluble

solubility

-

General description

Ionomycin is a calcium ionophore commonly derived from produced by the bacterium Streptomyces conglobatus, which means that it helps to transport calcium ions across cell membranes. It is a powerful tool for studying calcium signaling and its role in various biological processes, including cell activation, secretion, proliferation, and apoptosis.

Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.

Application

Ionomycin finds application in:
  • Study of calcium signaling and its role in various biological processes
  • Investigation of intracellular calcium levels, calcium-dependent enzymes, and cellular responses triggered by calcium influx
  • Induction of a rapid and controlled increase in intracellular calcium levels in cell biology experiments
  • Elucidation of specific cellular mechanisms and signaling pathways in combination with other substances, such as fluorescent calcium indicators or protein kinase activators
Ionomycin has been used:
  • to induce central demyelination
  • inhibit adrenal bovine TREK-1 channels
  • to regulate cell division of mature human B cells

Biochem/physiol Actions

Ionomycin is an ionophoric antibiotic that binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations.

Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes

Antimicrobial Spectrum: Active against Gram-Positive bacteria
Ionomycin is an ionophoric antibiotic which binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations. Ionomycin is more effective than A23187 as a mobile ion carrier for Ca2+. It is non-fluorescent. It is used to study Ca2+ transport across biological membranes and induces apoptotic degeneration of embryonic cortical neurons.

Features and Benefits

  • Ready available solution reduce the need for preparation time
  • Efficient choice for studying calcium signaling and its role in various biological processes
  • Allows for rapid and controlled manipulation of intracellular calcium levels
  • Versatile and widely used in cell biology research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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K M Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 148(6), 1797-1803 (1992-03-15)
The growth of a human B lymphoma cell line B104, an experimental model for mature B cells, was inhibited by ionomycin but not 12-O-tetradecanoylphorbol-13-acetate (TPA). Ionomycin inhibited B104 cells from entering into the M phase of the cell cycle without
K J Smith et al.
Brain : a journal of neurology, 117 ( Pt 6), 1351-1356 (1994-12-01)
The effects of injecting the calcium-selective ionophore, ionomycin, into myelinated tracts in the dorsal columns of adult rat spinal cords were examined electron microscopically. In vivo, ionomycin induced a primary vesicular demyelination, together with a variable degree of axonal degeneration
Thorsten Schäfer et al.
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Mast cells are secretory immune cells whose degranulation can provoke acute allergic reactions. It is presently unclear, however, whether an individual mast cell can repeatedly degranulate or turns dysfunctional after a single antigen stimulus. This work thus aims to better
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Bovine adrenocortical cells express bTREK-1 K(+) (bovine KCNK2) channels that are inhibited by ANG II through a Gq-coupled receptor by separate Ca(2+) and ATP hydrolysis-dependent signaling pathways. Whole cell and single patch clamp recording from adrenal zona fasciculata (AZF) cells
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British journal of pharmacology, 176(10), 1568-1584 (2018-08-04)
Duchenne muscular dystrophy (DMD), caused by dystrophin deficiency, results in chronic inflammation and irreversible skeletal muscle degeneration. Moreover, the associated impairment of autophagy greatly contributes to the aggravation of muscle damage. We explored the possibility of using non-euphoric compounds present

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