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I7260

Sigma-Aldrich

Monoclonal Anti-Human IgG3 antibody produced in mouse

clone HP-6050, ascites fluid

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Synonym(s):
Monoclonal Anti-Human IgG3
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6050, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:10,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
I6760B3523I5385
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

technique(s)

indirect ELISA: 1:10,000

technique(s)

indirect ELISA: 1:3,000

technique(s)

direct ELISA: 1:40,000

technique(s)

indirect ELISA: 1:5,000

isotype

IgG1

isotype

IgG2a

isotype

IgG1

isotype

IgG1

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Human IgG consists of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physicochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies.
Monoclonal Anti-Human IgG3 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Monoclonal Anti-Human IgG3 (I7260) is specific for the IgG3 subclass and does no react with IgG1, IgG2, and IgG4 in an ELISA. The IUIS/WHO12 study singled out this monoclonal antibody as one of the most widely applicable IgG3 specific monoclonal antibodies.
The antibody is specific for human IgG3 and is non-reactive with other IgG subclasses. This clone has been recommended by the IUIS/WHO study for its specificity and reactivity in a wide range of assay protocols and techniques.

Immunogen

Human IgG3
Purified human IgG3 myeloma proteins covalently coupled to poly aminostyrene (PAS) microbeads were used as the immunogen.

Application

Anti-Human IgG3 may be used for the identification of the IgG3 subclass by various immunoassays including enzyme-linked immunosorbent assay (ELISA), imprint immunofixation (IIF), immunofluorometric assay (IFMA), hemagglutination (HA), hemagglutination inhibition (HAI), particle counting immunoassay (PACIA), and detection of cytoplasmic IgG.

Biochem/physiol Actions

IgG3 exhibits protective action against wide range of intracellular bacteria, parasites and viruses. IgG3 antibodies potentially mediate various functions including, enhanced antibody-dependent cellular cytotoxicity (ADCC), opsonophagocytosis, complement activation and neutralization, compared with other IgG subclasses. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Role of IgG3 in Infectious Diseases
Damelang T, et al.
Trends in Immunology (2019)
Maternal human immunodeficiency virus-associated hypergammaglobulinemia reduces transplacental transfer of immunoglobulin G to Plasmodium falciparum antigens in Cameroonian neonates
Babakhanyan A, et al.
Open forum infectious diseases, 3(2), ofw092-ofw092 (2016)
Nasal application of rBet v 1 or non-IgE-reactive T-cell epitope-containing rBet v 1 fragments has different effects on systemic allergen-specific antibody responses.
Cornelia Egger et al.
The Journal of allergy and clinical immunology, 126(6), 1312-1315 (2010-08-03)
Patrick Waters et al.
Neurology(R) neuroimmunology & neuroinflammation, 2(3), e89-e89 (2015-03-31)
To optimize sensitivity and disease specificity of a myelin oligodendrocyte glycoprotein (MOG) antibody assay. Consecutive sera (n = 1,109) sent for aquaporin-4 (AQP4) antibody testing were screened for MOG antibodies (Abs) by cell-based assays using either full-length human MOG (FL-MOG)
Michael Graner et al.
PloS one, 15(2), e0228883-e0228883 (2020-02-23)
IgG oligoclonal bands (OCBs) are present in the cerebrospinal fluid (CSF) of more than 95% of patients with multiple sclerosis (MS), and are considered to be the immunological hallmark of disease. However, the target specificities of the IgG in MS

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